Effect of Fenton oxidized lignin support on immobilized β-glucosidase activity.

In this study, the Fenton oxidized lignin was prepared to investigate the effect of Fenton oxidation modification on the activity of lignin immobilized β-glucosidase (β-GL). The results demonstrated that Fenton oxidation could significantly improve the activity and stability of immobilized β-GL. This is because the Fenton oxidation increased the electrostatic, hydrogen bonding, and hydrophobic forces between lignin and β-GL, resulting in increased lignin adsorption onto β-GL. The Fenton oxidation also changed the chemical structure of lignin, altering the lignin-β-GL binding site and reducing the negative effect of lignin on the β-GL catalytic domain. This research will improve understanding of the effect of Fenton lignin oxidation on immobilized β-GL activity and expand the use of lignin in enzyme immobilization. [Display omitted] • Fenton oxidized lignin was a promising support for immobilization of β-GL. • Activity and stability of immobilized β-GL was improved by Fenton oxidated lignin. • Fenton oxidized lignin had strong interactions with β-GL. • Negative effect of lignin on β-GL catalytic domain was reduced by Fenton oxidation. [ABSTRACT FROM AUTHOR]