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Characterization of RING-type ubiquitin SINA E3 ligases and their responsive expression to salt and osmotic stresses in Brassica napus.

Authors :
Fang, Fengyan
Zhou, Wenlong
Liu, Yanfeng
Song, Zhizhong
Zheng, Songfeng
Wang, Fei
Lu, Zeyu
Qi, Dazhuang
Li, Bei
Sun, Nan
Tang, Xiaoli
Zhang, Juan
Zhan, Renhui
Wang, Limin
Zhang, Hongxia
Source :
Plant Cell Reports. May2023, Vol. 42 Issue 5, p859-877. 19p.
Publication Year :
2023

Abstract

SINA (Seven in absentia) proteins in the subtype of E3 ubiquitin ligase family play a crucial role in plant growth and development. However, their functions in response to salt and osmotic stresses in oil crops are still largely unknown. In this study, a total number of 23 BnaSINAs were identified in the rapeseed genome. Chromosome location and collinear relationship analyses revealed that they were unevenly distributed on 13 chromosomes, and have gone through 22 segmental duplication events under purifying selection. Phylogenetic and gene structural analyses indicated that they belonged to five main groups, and those in the same subgroup showed similar gene structure. All BnaSINAs were predicted to form homo- or heterodimers. Except BnaSINA7, BnaSINA11, BnaSINA17 and BnaSINA18, which lacked the N-terminal RING finger, all BnaSINAs contained a conserved C-terminal SINA domain, a typical structural feature of the RING-type E3 ligase family. Transcriptional expression analyses demonstrated that most BnaSINAs were ubiquitously expressed in roots, stems, leaves, flowers, pods and seeds, and all were responsive to salt and osmotic stresses. Further, yeast two-hybrid and Arabidopsis mutant complementation analyses demonstrated that BnaSINA4 interacted with BnaSINA17 to form heterodimer, and expression of BnaSINA17 in the Arabidopsissina2 mutant restored its growth resistance to salt and osmotic stresses. Our findings provide an important genetic foundation for the functional elucidation of BnaSINAs and a novel gene resource for the breeding of new oil crop cultivars with improved abiotic stress resistance. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
07217714
Volume :
42
Issue :
5
Database :
Academic Search Index
Journal :
Plant Cell Reports
Publication Type :
Academic Journal
Accession number :
163522953
Full Text :
https://doi.org/10.1007/s00299-023-02996-w