Anti- hyperglycaemic Effects of Avipattikara Churna and Triphla Churna in type -II diabetic Wistar Rats.

A Objective: The aim of the present study is to formulate a polyherbal formulation whose formula is given in Ayurvedic Formulary of India and evaluate its antidiabetic potential in animals. Method: The polyherbal formulation was prepared using the methanol extracts and aqueous extracts of the given parts of herbs in AFI. The quality of the finished product was evaluated as per the World Health Organization's guidelines for the quality control of herbal materials. Acute toxicity study was performed for dose selection as per OECD 423 guidelines. The quality testing parameters of the polyherbal formulation were within the limits. Results: The acute toxicity studies of the polyherbal formulation did not show any toxic symptoms in doses up to 2000 mg/kg over 14 days. The extracts of both the formulations were evaluated for In-vitro and In-vivo anti-diabetic study. The oral anti-diabetic activity of the polyherbal formulation (200 and 400 mg/kg, p.o) was screened against intraperitoneal (i.p.) STZ (35 mg/kg) 15 min after i.p. administration of nicotinamide (200 mg/kg) continuously for 2 days, to induced diabetes mellitus in Wistar rats. The investigational drug was administered for 28 consecutive days, and the effect of the polyherbal formulation on blood glucose levels, lipid profile, body weight and Oral Glucose tolerance was studied at regular intervals. At the end of the study, the blood samples were collected from all the animals for biochemical estimation, and the animals were sacrificed, and the kidney, liver and pancreatic tissues were collected for histopathologic analysis. Conclusion: Polyherbal formulations showed significant antidiabetic activity at 200 and 400 mg/kg, respectively, and this effect was comparable with that of Glibenclamide. [ABSTRACT FROM AUTHOR]