Broad-specificity monoclonal antibody against neonicotinoid insecticides via a multi-immunogen strategy and development of a highly sensitive GNP-based multi-residue immunoassay in ginseng and tomato.

[Display omitted] • A novel multi-immunogen strategy was proposed for mAb against NNIs. • A broad-specificity mAb against 8 NNIs with different structure types was prepared. • Recognition mechanism of the broad-specificity mAb was studied by molecular docking. • A sensitive GNP-based immunoassay was developed for ginseng and tomato samples. Neonicotinoid insecticides (NNIs) are extensively used across the agricultural products and foods. In order to meet the rapid detection requirements, a novel broad-specificity monoclonal antibody against NNIs was developed for the first time using a multi-immunogen strategy. The antibody's high affinity and its ability to bind target molecules were verified by ic-ELISA. Furthermore, molecular docking was used to evaluate the pivotal forces affecting binding affinity and to determine binding sites. Subsequently, a highly sensitive gold nanoparticle-based immunochromatographic assay was established for the rapid detection of eight NNIs and the IC 50 values were 0.03–1.61 ng/mL. The limits of detection for ginseng and tomato ranged from 0.76 to 30.19 μg/kg and 0.87 to 31.57 μg/kg, respectively. The spiked recovery ranged from 72.04% to 120.74%, and the coefficient of variation were less than 9.0%. This study provides a new direction for the development of multiple NNIs residue immunoassays. [ABSTRACT FROM AUTHOR]