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Peptide-based PET imaging agent of tumor TIGIT expression.
- Source :
-
EJNMMI Research . 5/2/2023, Vol. 13 Issue 1, p1-8. 8p. - Publication Year :
- 2023
-
Abstract
- Background: Accumulating studies have demonstrated that elevated TIGIT expression in tumor microenvironment correlates with better therapeutic response to TIGIT-based immunotherapy in pre-clinical studies. Therefore, a non-invasive method to detect tumor TIGIT expression is crucial to predict the therapeutic effect. Methods: In this study, a peptide-based PET imaging agent, 68Ga-DOTA-DTBP-3, was developed to non-invasively detect TIGIT expression by micro-PET in tumor-bearing BALB/c mice. DTBP-3, a D-peptide comprising of 12 amino acids, was radiolabeled with 68Ga through a DOTA chelator. In vitro studies were performed to evaluate the affinity of 68Ga-DOTA-DTBP-3 to TIGIT and its stability in fetal bovine serum. In vivo studies were assessed by micro-PET, biodistribution, and immunohistochemistry on tumor-bearing BALB/c mice. Results: The in vitro studies showed the equilibrium dissociation constant of 68Ga-DOTA-DTBP-3 for TIGIT was 84.21 nM and its radiochemistry purity was 89.24 ± 1.82% in FBS at 4 h in room temperature. The results of micro-PET, biodistribution and immunohistochemistry studies indicated that 68Ga-DOTA-DTBP-3 could be specifically targeted in 4T1 tumor-bearing mice, with a highest uptake at 0.5 h. Conclusion: 68Ga-DOTA-DTBP-3 holds potential for non-invasively detect tumor TIGIT expression and for timely assessment of the therapeutic effect of immune checkpoint blockade. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 2191219X
- Volume :
- 13
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- EJNMMI Research
- Publication Type :
- Academic Journal
- Accession number :
- 163449033
- Full Text :
- https://doi.org/10.1186/s13550-023-00982-7