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Preparation and characterization of nanobodies targeting SARS-CoV-2 papain-like protease.
- Source :
-
Protein Expression & Purification . Jul2023, Vol. 207, pN.PAG-N.PAG. 1p. - Publication Year :
- 2023
-
Abstract
- Coronavirus Papain-like protease (PLpro) mediates the cleavage of viral polyproteins and assists the virus escaping from innate immune response. Thus, PLpro is an attractive target for the development of broad-spectrum drugs as it has a conserved structure across different coronaviruses. In this study, we purified SARS-CoV-2 PLpro as an immune antigen, constructed a nanobody phage display library, and identified a set of nanobodies with high affinity for SARS-CoV-2. In addition, enzyme activity experiments demonstrated that two nanobodies had a significant inhibitory effect on the PLpro. These nanobodies should therefore be investigated as candidates for the treatment of coronaviruses. • A nanobody phage library of SARS-CoV-2 papain-like protease (PLpro)was successfully constructed. • The nanobodies were successfully purified and demonstrated to have high affinity for SARS-CoV-2 PLpro. • The nanobodies have a significant inhibitory effect on SARS-CoV-2 PLpro. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10465928
- Volume :
- 207
- Database :
- Academic Search Index
- Journal :
- Protein Expression & Purification
- Publication Type :
- Academic Journal
- Accession number :
- 163422562
- Full Text :
- https://doi.org/10.1016/j.pep.2023.106267