Anti-cancer activity of green synthesized silver nanoparticles using Ardisia gigantifolia leaf extract against gastric cancer cells.

Using extracts from herbs for silver nanoparticle synthesis is attracting attention for its anticancer activity. Ardisia gigantifolia is a herb used in traditional Chinese medicine for treating stomach ailments, and some compounds isolated from this plant exhibit the inhibitory activity against different cancer cells. However, the synthesis of silver nanoparticle using extract of Ardisia gigantiflia leaves and their anti-cancer activity was not reported. In this report, the green synthesized silver nanoparticles using Ardisia gigantiflia extract (Arg-AgNPs) has average diameter of 6 nm with functional groups including O–H, C–H, and C O founded on the surface of these nanoparticles. The viability assays results revealed Arg-AgNPs reduced gastric cancer cell proliferation in a dose-dependent manner, with IC 50 values of 1.37 and 0.65 μg/mL for AGS cells and 1.03 and 0.96 μg/mL for MKN45 cells. Arg-AgNPs caused cell cycle arrest at the G0/G1 phase and suppressed cell migration. Additionally, Arg-AgNPs significantly increased the percentage of senescent cells and promoted overproduction of reactive oxygen species (ROS) compared to the control. Thus, this study indicates that Arg-AgNPs can be considered as a promising candidate against human gastric cancer cells. • Arg-AgNPs were characterized by UV-Vis, XRD, TEM and FTIR. • Arg-AgNPs reduce cell viability and cause cell cycle arrest at G0/G1 phase in gastric cancer. • Arg-AgNPs reduce cell migration ability and induce cellular senescence in gastric cancer. • Arg-AgNPs promote overproduction of ROS in gastric cancer. [ABSTRACT FROM AUTHOR]