Tipificación molecular y susceptibilidad in vitro de aislamientos clínicos costarricenses del complejo Trichophyton mentagrophytes.

OBJECTIVE: To characterize Costa Rican clinical isolates of the T. mentagrophytes complex to determine the predominant species and their susceptibility pattern. MATERIALS AND METHODS: An experimental study was conducted to typify and determine the antifungal susceptibility of isolates from the T. mentagrophytes complex, obtained from skin and nail samples, done from 2019 to 2020. Molecular typification was performed by sequencing the ITS region. Broth microdilution M38-A method, as described by the Clinical Laboratory and Standards Institute, was employed to determine the antifungal susceptibility patterns of the isolates. RESULTS: Fifteen isolates were analyzed and all were identified as Trichophyton interdigitale. The MIC50 and MIC90 were 0.13 and 0.50 μg/mL for amorolfine, 0.25 and 0.50 μg/mL for bifonazole, 0.50 and 5.60 μg/mL for ciclopirox, 0.25 and 6.70 μg/mL. for itraconazole, 32 and 89.60 μg/mL for fluconazole, and 0.13 and 0.35 μg/mL for terbinafine respectively. CONCLUSIONS: In the present study, only T. interdigitale was identified among the species belonging to T. mentagrophytes complex, which demonstrates a person-toperson transmission. All isolates were susceptible to amorolfine, bifonazole, ciclopirox and terbinafine. However, the majority exhibited resistance to itraconazole and fluconazole. [ABSTRACT FROM AUTHOR]