Assessment of Radiolabeled L-Carnitine for Hepatotoxicity Imaging in Rats.

The current research was performed to assess the localization of radiolabeled Carnitine (CARNT) in different organs of rats. Radiolabeling of Carnitine (CARNT) was performed using Tc-99m with reasonable purity of 96.7±2.21% at the optimum conditions. The radiochemical purification of the 99mTc-CARNT was achieved by the TLC tool in two solvents and confirmed by the electrophoresis procedure. The 99mTc-CARNT was injected into three groups of rats. These rats were classified into three groups: the first one is a control group, the second was a pretreated group which was the toxicity group primarily treated by inactive Carnitine, and the last group is the toxicity rats group. The toxicity of rats was induced by Trichloroacetic Acid (TCA). The biochemical markers were done to describe the impacts of TCA on the enzymes and lipids of the rats. The accumulation of 99mTc-CARNT was examined at various times from 15.00 min to 120.00 min in three groups of rats (control, pretreated, and toxicity rats). The results explained that the 99mTc-CARNT was accumulated in the hepatotoxicity liver which was induced by the TCA higher than in control and pretreated rats. Hence, the data exhibited that the 99mTc-CARNT may be helpful for the determination of hepatocyte dysfunction in rats. [ABSTRACT FROM AUTHOR]