Effect of mannan oligosaccharides-Clostridium butyricum on fecal flora of 14-day-old broilers.

The experiment was conducted to investigate the effect of Clostridium butyrate, mannooligosaccharides and their combined addition on intestinal flora structure of broilers. Four hundred one-day-old healthy Kebao broilers with similar body weight were randomly divided into four groups, with five replicates in each group and 20 broilers in each replicate. Broilers in control group were fed with a basal diet, Clostridium butyricum group was fed with a basal diet supplemented with 2×109 CFU Clostridium butyricum, mannooligosaccharides group was fed with a basal diet supplemented with 0.2% mannooligosaccharides, combined group was fed with a basal diet supplemented with 2×109 CFU Clostridium butyricum + 0.2% mannooligosaccharides. The experiment lasted for 14 d. Compared with control group, the bacterial content of Firmicutes in each experimental group decreased, and the content of Proteobacteria increased. The content of Bacteroidetes in the combined addition group and the Clostridium butyricum group increased by 13.29% and 2.37%, respectively. Actinobacteria in Clostridium butyricum group was 2.89% higher than that in control group. Compared with control group, Bacilli content in all experimental groups decreased, while Gammapro-teobacteria and Clostridiales content increased. Compared with control group, the content of Lactobacillales decreased and the content of Enterbacteriales increased in all experimental groups. Compared with control group, the content of Lactobacillaceae decreased and the content of Enterobacteriaceae increased in all experimental groups. Compared with control group, the fecal Lactobacillus of broilers in all experimental groups decreased and Escherichia-Shigella increased. The content of Bacteroides in combined addition group and Clostridium butyricum group increased, while the content of mannooligosaccharides group decreased. The bacterial content of Lactobacillus and Comamonas decreased in each test group. The experiment indicates that the addition of Clostridium butyricum, mannan oligosaccharide or combined addition of Clostridium butyricum, mannan oligosaccharide in broiler feed can significantly change the dominant flora at the five classification levels of phylum, class, order, family and genus, and regulate the structure of intestinal flora. [ABSTRACT FROM AUTHOR]