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Validated Bio-Analytical Method for Cinacalcet Concentrations in Rat Plasma by Reversed Phase-High Performance Liquid Chromatography- Photodiode Array Detector Method.
- Source :
-
Indian Journal of Pharmaceutical Sciences . Mar/Apr2023, Vol. 85 Issue 2, p83-92. 10p. - Publication Year :
- 2023
-
Abstract
- The goal of this work was to create and verify a simple reversed phase-high performance liquid chromatography-photodiode array detector-based method for quantifying cinacalcet in rat plasma. Six albino Wistar rats were given cinacalcet orally by oral gavage. Blood was obtained at regular intervals using retro-orbital vein puncture and processed to get plasma. Cinacalcet was extracted from plasma using a 70:30 v/v ethanol:dichloromethane combination, with an 82.80 %-104.08 % recovery rate. The reversed phase-high performance liquid chromatography-photodiode array detector technique was used to determine cinacalcet plasma concentrations. The mobile phase was acetonitrile:0.1 M dipotassium phosphate buffer (pH 6.8) (50:50 v/v) at a flow rate of 1.0 ml/min. 241.0 nm was used as the detecting wavelength. The method's linearity for cinacalcet was proven by a correlation coefficient (R²) of more than 0.9996 and a linear range of 1 µg/ml to 24 µg/ml for cinacalcet. The lower and upper limits of quantification were found to be 1 µg/ml and 24 µg/ml respectively. At the cinacalcets retention time, no interfering peaks were seen. This reversed phase-high performance liquid chromatography-photodiode array detector approach is a straightforward and effective way to determine pharmacokinetic parameters by measuring plasma cinacalcet levels. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0250474X
- Volume :
- 85
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Indian Journal of Pharmaceutical Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 163153324