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MiR-125b regulates the differentiation of hair follicles in Fine-wool Sheep and Cashmere goats by targeting MXD4 and FGFR2.
- Source :
-
Animal Biotechnology . Apr2023, Vol. 34 Issue 2, p357-364. 8p. - Publication Year :
- 2023
-
Abstract
- With the development of miRNAs identification technology, more and more miRNAs have been discovered, and the role of miRNAs in the development of animal hair follicles has become a focus of research on hair-producing animals. In the previous experiment, compare the microRNA (miRNA) trancriptomes of goats and sheep skin using Solexa sequencing and differentially expressed miR-125b was screened. However, the mechanism of miR-125b regulating hair follicle development is not clear. Therefore, in the present study, the expression of miR-125b, MXD4 and FGFR2 in skin tissue of Fine-wool Sheep and Cashmere goats and HEK-293T cells was examined by qPCR and Western blot. Furthermore, the correlation between miR-125b and the predicted target gene (MXD4, FGFR2) was verified using the Dual-luciferase Reporter assay. We demonstrated that the expression of MXD4 and FGFR2 in Cashmere goats was significantly higher than that of Fine-wool Sheep, and the expression was opposite to that of miR-125b. miR-125b can down-regulate the levels of MXD4 and FGFR2. Dual-luciferase reporter gene assay showed that miR-125b could bind to the 3′-UTR region of target genes FGFR2 and MXD4, suggesting that MXD4 and FGFR2 were target genes of miR-125b. This study has shown that the growth and development of hair follicles in skin tissue of Fine-wool Sheep and Cashmere goats from the new regulatory levels of miRNAs, and clarified the mechanism of miR-125b and its target genes in the development of hair follicles in the skin. [ABSTRACT FROM AUTHOR]
- Subjects :
- *MICRORNA
*HAIR follicles
*SHEEP
*GOATS
*MERINO sheep
*CASHMERE
*REPORTER genes
Subjects
Details
- Language :
- English
- ISSN :
- 10495398
- Volume :
- 34
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Animal Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 163091366
- Full Text :
- https://doi.org/10.1080/10495398.2021.1968884