复方补肾活血颗粒含药血清通过Trb3调控hBMSCs成骨成脂分化.

Objective To investigate the regulation of osteogenic/adipogenic differentiation of human bone marrow mesenchymal stem cells via Trb3 through Compound Kidney-Invigorating Granules（CKG） medicated serum. Methods hBMSCs were intervened with different concentrations of CKG-medicated serum. Cell viability was measured by CCK8 assay, hBMSCs osteogenic differentitation was observed by ALP activity and alizarin red staining, and adipogenic differentiation by oil red O staining. hBMSCs were examined by Western blot for osteogenic/adipogenic marker protein expression. Western blot and qPCR were performed to defect Trb3 protein and gene expression. Trb3 siRNA was transfected with hBMSCs to observe the expression of osteogenic/adipogenic related factors by Western blot. Results CKG medicated serum promoted hBMSCs proliferation in a concentration-dependent manner. CKG medicated serum enhanced ALP activity and mineralized nodules. It upregulated Runx2 and Osterix protein expression, and inhibited PPARγ and FABP4 protein expression and lipid accumulation. Mechanistic studies revealed that Trb3 knockdown reversed the ability of CKG medicated serum to promote osteogenic differentiation and inhibit adipogenic differentiation. Conclusion CKG-medicated serum promotes osteogenic differentiation of hBMSCs via Trb3 at the expense of adipogenic differentiation. [ABSTRACT FROM AUTHOR]