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Rapid detection of three mycotoxins in animal feed materials using competitive ELISA-based origami microfluidic paper analytical device (μPAD).
- Source :
-
Analytical & Bioanalytical Chemistry . Apr2023, Vol. 415 Issue 10, p1943-1951. 9p. - Publication Year :
- 2023
-
Abstract
- We report the development of a competitive ELISA-based origami microfluidic paper-based analytical device (μPAD) for the detection of mycotoxins in animal feed material. The μPAD was patterned using the wax printing technique with the design of a testing pad in the middle and two absorption pads at the side. Anti-mycotoxin antibodies were effectively immobilized on chitosan–glutaraldehyde-modified sample reservoirs in the μPAD. The determination of zearalenone, deoxynivalenol, and T-2 toxin in corn flour was successfully achieved by performing competitive ELISA on the μPAD in 20 min. Colorimetric results were easily distinguished by the naked eye with a detection limit of 1 µg/mL for all three mycotoxins. The μPAD integrated with competitive ELISA holds potential for practical applications in the livestock industry for rapid, sensitive, and cost-effective detection of different mycotoxins in animal feed materials. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ANIMAL feeds
*MYCOTOXINS
*ORIGAMI
*ANIMAL industry
*PRINTMAKING
*CORN flour
Subjects
Details
- Language :
- English
- ISSN :
- 16182642
- Volume :
- 415
- Issue :
- 10
- Database :
- Academic Search Index
- Journal :
- Analytical & Bioanalytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 162727341
- Full Text :
- https://doi.org/10.1007/s00216-023-04612-y