Back to Search
Start Over
A ratiometric fluorescent probe for single and two-photon bioimaging of hydrogen peroxide in vitro and in vivo.
- Source :
-
Sensors & Actuators B: Chemical . Jun2023, Vol. 384, pN.PAG-N.PAG. 1p. - Publication Year :
- 2023
-
Abstract
- Hydrogen peroxide (H 2 O 2) as an important reactive oxygen species (ROS) is correlated in various oxidative stress and cell signaling processes. Thus, developing an effective method that can monitor H 2 O 2 in the Golgi apparatus is of great importance. Herein, a Golgi-targeting two-photon fluorescent probe (GT-H 2 O 2) that can be excited by a femto-second pulse (760 nm) is designed. GT-H 2 O 2 utilized aminoquinoline as a fluorophore / Golgi-targetable unit and borate ester as a H 2 O 2 -responsive unit with an intramolecular charge transfer strategy. The borate ester of GT-H 2 O 2 was oxidized by H 2 O 2 selectively to afford a new fluorescent molecule GT-NH 2 , thereby realizing a ratiometric detection of H 2 O 2. The probe exhibited a high sensitivity and selectivity to H 2 O 2 with a linear range from 5 to 60 μM. In addition, endogenous / exogenous H 2 O 2 could be directly visualized utilizing GT-H 2 O 2 in vitro and in vivo by one/two-photon microscopy. • A two-photon fluorescent probe (GT-H 2 O 2) is presented to sense H 2 O 2 levels. • GT-H 2 O 2 exhibited a rapid and notable ratiometric fluorescent response for H 2 O 2. • GT-H 2 O 2 can achieve Golgi localization. • GT-H 2 O 2 was visualized to monitor endogenous/exogenous H 2 O 2 by two-photon imaging. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09254005
- Volume :
- 384
- Database :
- Academic Search Index
- Journal :
- Sensors & Actuators B: Chemical
- Publication Type :
- Academic Journal
- Accession number :
- 162635732
- Full Text :
- https://doi.org/10.1016/j.snb.2023.133668