Propriétés antiseptiques d’extrait éthanolique de Juglans regia (L.) et évaluation de son potentiel antibiofilm in vitro en implantologie orthopédique.

The objective assigned to this study is to develop an antiseptic solution based on the ethanolic extract of the leaves of walnut (Juglans regia L.) and to evaluate the antibiofilm potential in vitro growth strains of staphylococci implicated in osteoarticular infections on the material (OAMI) in postoperative orthopedic surgery. The ethanolic extract of Juglans regia L. has been incorporated as an antimicrobial active principle in the formulation of the antiseptic solution 2%. Fourteen strains of Staphylococcus aureus were collected from the pus of postoperative wounds, from subjects suffering from a bone fracture, benefiting from a prosthesis and/or osteosynthesis material (external fixator, screw, nail, plate screwed). The Microtiter plate method was used to assess the ability of the pathogenic strains collected to produce the biofilm. The antibiofilm potential of the antiseptic solution 2% was achieved by the liquid dilution technique for the determination of the minimum inhibitory concentration of biofilm (CMIB). CMIB demonstrated a remarkable antibiofilm effect. A CMIB measured at 6.25% (v/v) showed a degree of antibiofilm sensitivity effective in vitro on 92.86% of strains. Faced with the effectiveness of the antiseptic solution 2% on the germs incriminated in OAMI, its use as a natural product without adverse effects for local care and postsurgical skin disinfection seems promising in the fight against infections. [ABSTRACT FROM AUTHOR]