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A Pilot Study Exploring Temporal Development of Gut Microbiome/ Metabolome in Breastfed Neonates during the First Week of Life.
- Source :
-
Pediatric Gastroenterology, Hepatology & Nutrition . Mar2023, Vol. 26 Issue 2, p99-115. 19p. - Publication Year :
- 2023
-
Abstract
- Purpose: Exclusive breastfeeding promotes gut microbial compositions associated with lower rates of metabolic and autoimmune diseases. Its cessation is implicated in increased microbiome-metabolome discordance, suggesting a vulnerability to dietary changes. Formula supplementation is common within our low-income, ethnic-minority community. We studied exclusively breastfed (EBF) neonates' early microbiome-metabolome coupling in efforts to build foundational knowledge needed to target this inequality. Methods: Maternal surveys and stool samples from seven EBF neonates at first transitional stool (0-24 hours), discharge (30-48 hours), and at first appointment (days 3-5) were collected. Survey included demographics, feeding method, medications, medical history and tobacco and alcohol use. Stool samples were processed for 16S rRNA gene sequencing and lipid analysis by gas chromatography-mass spectrometry. Alpha and beta diversity analyses and Procrustes randomization for associations were carried out. Results: Firmicutes, Proteobacteria, Bacteroidetes and Actinobacteria were the most abundant taxa. Variation in microbiome composition was greater between individuals than within (p=0.001). Palmitic, oleic, stearic, and linoleic acids were the most abundant lipids. Variation in lipid composition was greater between individuals than within (p=0.040). Multivariate composition of the metabolome, but not microbiome, correlated with time ( p=0.030). Total lipids, saturated lipids, and unsaturated lipids concentrations increased over time (p=0.012, p=0.008, p=0.023). Alpha diversity did not correlate with time (p=0.403). Microbiome composition was not associated with each samples' metabolome (p=0.450). Conclusion: Neonate gut microbiomes were unique to each neonate; respective metabolome profiles demonstrated generalizable temporal developments. The overall variability suggests potential interplay between influences including maternal breastmilk composition, amount consumed and living environment. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 22348646
- Volume :
- 26
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Pediatric Gastroenterology, Hepatology & Nutrition
- Publication Type :
- Academic Journal
- Accession number :
- 162534825
- Full Text :
- https://doi.org/10.5223/pghn.2023.26.2.99