柚皮苷对骨质疏松大鼠拔牙创骨愈合的影响.

BACKGROUND: Naringin, a monomer compound of Traditional Chinese medicine, has anti-inflammatory and osteogenic effects, but its effect on the healing rate of tooth extraction wounds is not clear. OBJECTIVE: To investigate the effect of naringin on the healing rate of tooth extraction wounds in rat models of osteoporosis. METHODS: Forty-eight female Sprague-Dawley rats were randomly divided into sham-operation group (n=14) and ovariectomized group (n=34). In the ovariectomized group, bilateral ovaries were removed to establish osteoporosis models. After 6 weeks, four rats in each group were randomly selected for MicroCT detection to confirm the modeling results. After successful modeling, the remaining rats were subject to tooth extraction and randomly divided into tooth extraction group (the remaining rats in the sham-operation group), control group, naringin group, and estradiol group, and the second molar of left maxillary was extracted. Naringin group was given naringin 300 mg/kg/d by gavage, estradiol group was given 17-β estradiol 20 μg/kg/d by subcutaneous injection, and tooth extraction group and control group were treated with the same volume of normal saline. After 4 weeks of intervention, the rat maxilla was separated for hematoxylin-eosin staining, Masson staining, and MicroCT detection. The expressions of osteoprotegerin and receptor activator of nuclear factor-κB ligand (RANKL) at mRNA and protein levels in the bone tissue around the tooth extraction wounds were also detected. RESULTS AND CONCLUSION: After 6 weeks of castration, the bone volume fraction of the ovariectomized group decreased, indicating that the modeling of osteoporosis was successful. Four weeks after tooth extraction, compared with the control group, the naringin group and estradiol group had smaller trabecular separation and less loss of alveolar bone height, indicating that naringin could promote bone healing of tooth extraction wounds. Compared with the control group, the expression level of osteoprotegerin and RANKL in bone tissues around tooth extraction was increased and decreased respectively in the naringin and estradiol groups, indicating that naringin could up-regulate osteoprotegerin expression and down-regulate RANKL expression. [ABSTRACT FROM AUTHOR]