ANTIOXIDANT AND HEPATOPROTECTIVE ACTIVITY OF EPHEDRA ALATA EXTRACTS AGAINST INTOXICATION WITH DELTAMETHRIN PESTICIDE IN MALE RATS.

The objective of this study is to evaluate the antioxidant and hepatoprotective activity of Ephedra alata alenda plant, against intoxication with Deltamethrin pesticide in rats. After preparation of plant extracts (macerated and decocted, powder), chemical profile and total phenolic compounds of plant were estimated. Furthermore, antioxidant activities In vitro were evaluated using the DPPH test, and to verify the antitoxic activity In vivo, male rats were exposed to Delamethrin (3.5 µl/kg/day) for 30 days and then treated for 14 days with plant preparations. The fourth group of rats is the positive control group exposed just to Delthmethrin, while the fifth group is the negative control group. 44 days later, rats were sacrificed, Blood samples were collected for serum biochemical estimation, and liver fragments were reserved to assess antioxidant activity. Results of plant extracts showed the presentation of many natural substances in two extracts. The concentration of total phenolic compounds was estimated at 13.72 mg EAG/g, 13.45 mg EAG/g for macerated and decocted extracts respectively. DPPH test was estimated at IC50=0.901 mg/ml and IC50=1.141 mg/ml for macerated and decocted extracts, respectively. Antioxidant activity results In vivo showed significant differences in blood biochemical such as ALT, ALP, Bilirubin and Urea, oxidative stress parameters (GSH), (GST), and lipid peroxidation (MDA), noting the reforms in the sections of some liver tissues that demonstrated the effectiveness of plant extract compared and its absence completely in the tissue of the liver treated with the powder. Finally, the liver tissue sections was confirmed with biochemical results. [ABSTRACT FROM AUTHOR]