Applications Of The Immune-Histochemical Technique For Diagnosis Of Helicobacter Pylori From Gastric Biopsy Samples.

The Helicobacter pylori have ancestral relation with the family of Proteobacteria, order Campylobacterales, family Helicobacteraceae. It is the first and most important cause of gastric carcinoma along with peptic ulcer and dyspepsia carcinoma. H. pylori is also responsible for mucosa associated with lymphoid tissue (MALT) and Known Hodgkin Lymphoma. It is assessed that patients having H. pylori are a 30% to 40% possibility of evolving gastric ulcer and a 10% to 20% possibility of developing distal gastric cancer. It causes chronic inflammation and significantly increases the risk of duodenal, gastric ulcer, and cancer. It is the second leading cause of cancer-related deaths in the world. In the current study, a total of 210 gastric biopsy specimens were collected from the patient with a history of gastritis from Chughtai Lab in 10% NBF. All tissue size was measured and gross examined these samples were processed in an automated tissue processor Tissue-Tek VIP VI (Japan). After processing, the embedding of tissues was done in paraffin wax at Tissue-Tek TEC. 2- 3 µm sections were prepared using rotary microtome Leica 201 USA. A comparison was carried out between H&E and IHC. Helicobacter pylori were detected in 88 cases out of 200 while 09 samples were either poorly preserved or autolyzed that's why further processing and diagnosis were not possible. One case was diagnosed as poorly differentiated adenocarcinoma; the sample was collected from a female patient of 56 years. Out of 88 positive cases, 66 (75%) were initially screened as positive for H.pyloriby H&E staining due to the presence of mild or moderate colonization while the remaining 25% cases were negative by H&E staining but with suspicion of neutrophils and lymphoid aggregates. All 88 cases were further confirmed by IHC. Our findings confirmed that IHC is a batter reliable and more accurate method in the diagnosis of H.pylori and it should be considered the gold standard. [ABSTRACT FROM AUTHOR]