Whole genome sequence analysis enabled affirmation of the probiotic potential of marine sporulater Bacillus amyloliquefaciens BTSS3 isolated from Centroscyllium fabricii.

[Display omitted] • Bacillus amyloliquefaciens BTSS3, is a marine spore former isolated from deep-sea shark Centroscyllium fabricii. • Whole genome sequencing of BTSS3 was done using the Illumina Hiseq2500 platform. • In-depth automated annotation of CAZymes using dbcan CAZyme database revealed the prescence of enzymes important in complex carbohydrate metabolism. • Functional annotation of genome showed the presence of probiotic genes related to the biosynthesis of bacteriocins, stress resistance, and bile tolerance. • Colonization of fluorescent- labelled Bacillus amyloliquefaciens BTSS3 in zebrafish (Danio rerio) gut confirms its ability to adhere to gut mucosa. Probiotics are microorganisms when administered in adequate amounts, confer health benefits on the host. Many probiotics find application in various industries however, probiotic bacteria linked to marine environments are less explored.Although Bifidobacteria, Lactobacilli , and Streptococcus thermophilus are the most frequently used probiotics, Bacillus spp. have acquired much acceptance in human functional foods due to their increased tolerance and enduring competence in harsh environments like the gastrointestinal (GI) tract. In this study, the 4 Mbp genome sequence of Bacillus amyloliquefaciens strain BTSS3, a marine spore former isolated from deep-sea shark Centroscyllium fabricii , with antimicrobial and probiotic properties was sequenced, assembled, and annotated. Analysis revealed the presence of numerous genes presenting probiotic traits like production of vitamins, secondary metabolites, amino acids, secretory proteins, enzymes and other proteins that allow survival in GI tract as well as adhesion to intestinal mucosa. Adhesion by colonization in the gut was studied in vivo in zebrafish (Danio rerio) using FITC labelled B.amyloliquefaciens BTSS3. Preliminary study revealed the ability of the marine Bacillus to attach to the intestinal mucosa of the fish gut. The genomic data and the in vivo experiment affirms that this marine spore former is a promising probiotic candidate with potential biotechnological applications. [ABSTRACT FROM AUTHOR]