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miR-330-3p alleviates the progression of atherosclerosis by downregulating AQP9.
- Source :
-
Functional & Integrative Genomics . Jun2023, Vol. 23 Issue 2, p1-11. 11p. - Publication Year :
- 2023
-
Abstract
- Atherosclerosis (AS) is the main cause of cardiovascular diseases. However, the role of AQP9 in AS is not well understood. In the present study, we predicted that miR-330-3p might regulate AQP9 in AS through bioinformatics analysis, and we established AS model using ApoE−/− mouse (C57BL/6) with high-fat diet (HFD). Hematoxylin and eosin (H&E) and Oil red O staining were used to determine atherosclerotic lesions. CCK8 and Ethyny1-2-deoxyuridine (EdU) assays were used to investigate human umbilical vein endothelial cells (HUVECs) proliferation after treatment with 100 μg/mL ox-LDL. Wound scratch healing and transwell assays were used to measure the cell invasion and migration ability. Flow cytometry assay was used to determine apoptosis and cell cycle. A dual-luciferase reporter assay was performed to investigate the binding of miR-330-3p and AQP9. We identified that the expression of miR-330-3p in AS mice model decreased while the expression level of AQP9 increased. miR-330-3p overexpression or down-regulation of AQP9 could reduce cell apoptosis, promote cell proliferation, and migration after ox-LDL treatment. Dual-luciferase reporter assay result presented that AQP9 was directly inhibited by miR-330-3p. These results suggest that miR-330-3p inhibits AS by regulating AQP9. miR-330-3p/AQP9 axis may be a new therapeutic target for AS. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 1438793X
- Volume :
- 23
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Functional & Integrative Genomics
- Publication Type :
- Academic Journal
- Accession number :
- 162259842
- Full Text :
- https://doi.org/10.1007/s10142-023-01001-7