SPP1在头颈部鳞状细胞癌的免疫浸润及临床相关性分析.

The correlation between secreted phosphoprotein 1 (SPPf) and immune infiltration and the clinical correlation of SPP1 in head and neck squamous cell carcinoma(HNSC) were analyzed, and the potential value of SPP1 in the prognosis and individualized treatment of HNSC was clarified. The HNSC data from The Cancer Genome Atlas(TCGA) was used to analyze SPP1 expression. The clinical survival data from TCGA was used to evaluate the clinical prognostic value of SPP1. The clusterProfiler package of R language was applied for the enrichment analysis related to SPP1. The CIBERSORT function of R language was used to evaluate the infiltration of 22 kinds of tumor infiltrating immune cells in HNSC. Subsequently, the correlation analysis between tumor infiltrating immune cells and SPP1 expression was performed. Differential expression analysis found that SPP1 was highly expressed in HNSC (PcO.OOl). Clinical correlation analysis found that SPP1 expression was correlated with T stage (P = 0.001) and clinical stage (P = 0.013). The overall survival of patients with high SPP1 expression was significantly shorter than the patients with low SPP\expression (P = 0.020 4). Gene enrichment analysis found that SPP1 was associated with immunological functions and immune-related pathways in HNSC. Analysis of tumor infiltrating immune cells found that in the high SPP1 expression group, the infiltration proportions of M2 macrophages (P = 0.001 1), resting dendritic cells (P = 0.005 5), and activated mast cells (P = 0.048 8) increased, while the infiltration proportions of actived memory CD4+ T lymphocytes (P<0.001), plasma cells (P = 0.026 6), and resting mast cells (P = 0.038 6) decreased. The research results indicate that SPP1 acts as an oncogene in HNSC and is related to the clinical outcome of patients. SPPl may play an important role in the tumor immune microenvironment and may become a valuable prognostic biomarker and immunotherapy target in HNSC. [ABSTRACT FROM AUTHOR]