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Starch granular size and multi-scale structure determine population patterns in bivariate flow cytometry sorting.
- Source :
-
International Journal of Biological Macromolecules . Mar2023, Vol. 231, pN.PAG-N.PAG. 1p. - Publication Year :
- 2023
-
Abstract
- Bivariate flow cytometry (FC) sorting with forward scatter (FSC) and side scatter (SSC) is a recently established novel technique to separate starch granules. However, the forming mechanism of starch FC-dependent population patterns (i.e. the number of subgroups (NS) and FSC/SSC-dependent distribution patterns) remain partly elusive. For this, the correlation of granular size and multi-scale structure of native starches and FC-dependent population patterns was investigated through employing a wide range of native starches originating from different species involving cereal-, pulse-, and tuber crops. Results showed NS was pertinent with particle size, amylose content (AC), amylopectin chains length distribution, lamellar structure, short-range ordered structure. The distinct NS was determined by impacts of native starch FSC / SSC-dependent distribution patterns. Specifically, starch granular size significantly correlated with both FSC and SSC-dependent distribution patterns. The proportion of chains with DP 6–12 was the intra-molecular decisive factor to influence short-range ordered structure, finally leading to FSC-dependent distribution patterns. By contrast, AC was another intra-molecular index to determine SSC-dependent distribution patterns through affecting lamellar structure and short-range ordered structure. • Side scatter (SSC) is vulnerable to short-range ordered and lamella structures. • Forward scatter (FSC) is sensitive to short-range ordered structure. • Starch particle size affect distribution patterns of both FSC and SSC. • The correlation of starch features and FSC/SSC exists in limited range. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01418130
- Volume :
- 231
- Database :
- Academic Search Index
- Journal :
- International Journal of Biological Macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 161953528
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2023.123306