OCENA PRZYDATNOŚCI IN VITRO OPATRUNKU LIPIDOKOLOIDOWEGO ZAWIERAJĄCEGO JONY SREBRA DO ERADYKACJI BIOFILMÓW MIESZANYCH PSEUDOMONAS AERUGINOSA I STAPHYLOCOCCUS AUREUS TWORZĄCYCH SIĘ W ZRÓŻNICOWANYCH WARUNKACH HODOWLANYCH.

Introduction The presence of biofilms in a non-healing wound is considered one of the most significant factors complicating the healing process and increasing the risk of infection transition from the local to the generalized (systemic) form. The management of complications associated with the presence of biofilm infection may include such modalities as the use of dressings designed to eradicate these microbial communities, as well as to control the exudate level. Aim In this in vitro experimental research, the applicability of lipocolloid dressings containing silver ions for the eradication of mixed biofilms of Pseudomonas aeruginosa and Staphylococcus aureus cultures was assessed. Material and methods Strains cultured on an organic carrier resembling the wound surface were assessed, as well as in fluids simulating the non-healing wound environment. Results The obtained results demonstrated a high differentiation of the number of cells forming the biofilm in the specific culturing fluids and a significantly higher component of P. aeruginosa cells than S. aureus in the biofilm structure. The applied dressings were characterized by a high eradication capacity of the mixed biofilm of P. aeruginosa and S. aureus, with full eradication of this structure observed in the artificial exudate. The dressings were able to absorb 6 to 9 times their dry weight, depending on the wound-simulating fluid used, and they did not lose their cohesiveness when transformed into a gel form. Conclusion On the basis of the obtained results, it should be emphasized that lipocolloid dressings containing silver ions are characterized by a high ability to eradicate the mixed biofilm of P. aeruginosa and S. aureus and present a high ability to control the exudate level. [ABSTRACT FROM AUTHOR]