The development of microfluidic-based western blotting: Technical advances and future perspectives.

• A critical overview of advances in microfluidic-based western blotting, highlighting advantages and disadvantages of each approach. • Particular focus on the development of now commercially available systems, including those for single cell analysis. • Includes recent developments in algorithms for automation, materials, projection electrophoresis and triBlots. • Future potential for use in point-of-care diagnostics is also discussed. Over the past two decades significant technical advancement in the field of western blotting has been made possible through the utilization of microfluidic technologies. In this review we provide a critical overview of these advancements, highlighting the advantages and disadvantages of each approach. Particular attention is paid to the development of now commercially available systems, including those for single cell analysis. This review also discusses more recent developments, including algorithms for automation and/or improved quantitation, the utilization of different materials/chemistries, use of projection electrophoresis, and the development of triBlots. Finally, the review includes commentary on future advances in the field based on current developments, and the potential of these systems for use as point-of-care devices in healthcare. [ABSTRACT FROM AUTHOR]