Back to Search
Start Over
Visualizing the Intermittent Gating of Na+/H+ Antiporters in Single Native Bioluminescent Bacteria**.
- Source :
-
Angewandte Chemie International Edition . 2/13/2023, Vol. 62 Issue 8, p1-7. 7p. - Publication Year :
- 2023
-
Abstract
- While the intermittent gating of ion channels has been well studied for decades, dynamics of the action of secondary transporters, another major pathway for ion transmembrane transports, remains largely unexplored in living cells. Herein, intermittent blinking of the spontaneous bioluminescence (BL) from single native bacteria, P. phosphoreum, was reported, investigated and attributed to the intermittent gating of sodium/proton antiporters (NhaA) between the active and inactive conformations. Each gating event caused the rapid depolarization and recovery of membrane potential within several seconds, accompanying with the apparent BL blinking due to the transient inhibitions on the activity of the respiratory chain. Temperature‐dependent measurements further obtained an activation energy barrier of the conformational change of 20.3 kJ mol−1. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ION channels
*ACTIVATION energy
*MEMBRANE potential
*BIOLUMINESCENCE
Subjects
Details
- Language :
- English
- ISSN :
- 14337851
- Volume :
- 62
- Issue :
- 8
- Database :
- Academic Search Index
- Journal :
- Angewandte Chemie International Edition
- Publication Type :
- Academic Journal
- Accession number :
- 161757746
- Full Text :
- https://doi.org/10.1002/anie.202215800