Preparation and identification of isoquinoline alkaloids with ATP citrate lyase inhibitory activity from Dactylicapnos scandens.

Three new isoquinoline alkaloids including a morphine derivative (1), two aporphine alkaloids (2 – 3), together with five known alkaloids (4 – 8) were obtained from the extract of Dactylicapnos scandens (D.Don) Hutch. (D. scandens). Their structures and absolute configurations were elucidated by extensive spectroscopic data analysis including HRESIMS, NMR and electronic circular dichroism (ECD) and ECD calculation. Compounds 1 – 8 were evaluated for ATP Citrate Lyase (ACLY) inhibitory activity through an enzymatic assay. Among them, 2 and 3 showed the high ACLY inhibitory activity with an IC 50 value of 10.48 ± 1.59 and 10.89 ± 4.89 μM. [Display omitted] • Three new alkaloids were obtained from Dactylicapnos scandens. • Their structures were evaluated by the analysis of extensive spectroscopic data. • Compound 2 and 3 exhibited significant ACLY inhibitory activity. [ABSTRACT FROM AUTHOR]