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Characteristics of glucose oxidase immobilized on carbon-encapsulated iron nanoparticles decorated with polyethyleneimine.
- Source :
-
Polymer Bulletin . Feb2023, Vol. 80 Issue 2, p1565-1586. 22p. - Publication Year :
- 2023
-
Abstract
- This study aimed to conjugate glucose oxidase (GOX) with carbon-encapsulated iron nanoparticles (Fe@C) which were functionalized with carboxylic groups and decorated with branched polyethyleneimine (PEI), in order to yield Fe@C-NH-PEI-GOX and Fe@C-(CH2)2-CONH-PEI-GOX derivatives. The process of electrostatic attachment of the GOX enzyme on the Fe@C-NH-PEI and Fe@C-(CH2)2CONH-PEI constructs was optimized, and the amount of enzyme attached on the constructs was also estimated. The steady-state kinetics of β-D-glucose oxidation by molecular oxygen, a reaction catalyzed by GOX, were compared using a native GOX enzyme obtained from Aspergillus niger and nanoparticles-conjugated GOX. The Fe@C-NH-PEI-GOX and Fe@C-(CH2)2-CONH-PEI-GOX constructs were formed by electrostatic interaction between positively charged Fe@C-NH-PEI and Fe@C-(CH2)2-CONH-PEI and negatively charged GOX in water media at pH 6. The activity of the immobilized enzyme on Fe@C-NH-PEI-GOX and Fe@C-(CH2)2-CONH-PEI-GOX derivatives was preserved, with Km values of 3271 and 1605 µM, respectively. However, the catalytic activity of Fe@C-NH-PEI-GOX and Fe@C-(CH2)2-CONH-PEI-GOX was reduced up to 20- and tenfold in comparison to that of the free GOX enzyme. The results of the study suggest that PEI is a promising cationic polymer with a high loading capacity and that the use of a simple adsorption process for preparing Fe@C-NH-PEI-GOX and Fe@C-(CH2)2-CONH-PEI-GOX conjugates is a highly efficient approach for stable immobilization of GOX enzyme on carbon-encapsulated iron nanoparticles. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01700839
- Volume :
- 80
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Polymer Bulletin
- Publication Type :
- Academic Journal
- Accession number :
- 161550737
- Full Text :
- https://doi.org/10.1007/s00289-022-04125-1