Leflunomid Akut Yüksek Fruktoz ile Beslenen Sıçanların Hipotalamusunda Lipid Profillerini ve Oksidatif Stresi İyileştirir.

This study aimed to investigate the effects of oxidative stress and lipid profiles on the hypothalamus of rats fed with acute high-dose fructose and the possible contribution of leflunomide to these effects. Totally 48 Spraque–Dawley male rats 12-16 weeks old, weighing 250-300 g were included and were randomly divided into 6 groups; group 1: control, group 2: carboxymethyl cellulose+control, group 3: leflunomide, group 4: acute high-dose fructose, group 5: acute highdose fructose+carboxymethyl cellulose, and group 6: acute high-dose fructose+leflunomide. Acute high-dose fructose groups were given 63% fructose solution for 24 hours after 48 hours of fasting. Since leflunomide is not soluble in water, it was dissolved in 1% carboxymethyl cellulose solution and 10 mg/kg/day of leflunomide was administered orally in the morning and evening. After 72 hours, the rats were sacrificed under general anesthesia and the hypothalamus tissue was removed. The low-density lipoprotein (LDL), total cholesterol, total antioxidant-oxidant status (TAS-TOS), and oxidative stress index (OSI) levels were determined from the obtained hypothalamus tissue using commercial kits based on enzyme-linked immunosorbent assay method. There was no significant difference between the groups in total antioxidant level. A significant increase was found in the TOS and OSI levels in fourth group vs first group (P <0.001). In the treatment groups, a statistically significant decrease was observed with leflunomide (P <.05). A statistically significant decrease was detected in LDL and total cholesterol levels in sixth group versus fourth group (P <.05). In this study, the importance of Leflunomide in reducing oxidative stress and lipid profile in high fructose-fed rats was determined. In conclusion, Leflunomide may be promising in reducing brain damage due to oxidative stress. [ABSTRACT FROM AUTHOR]