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Cyanate Degradation in Different Matrices Using Heat-Purified Enzymes.
- Source :
-
Catalysts (2073-4344) . Jan2023, Vol. 13 Issue 1, p76. 7p. - Publication Year :
- 2023
-
Abstract
- A green and low-cost removal method for cyanate, a toxic byproduct from the treatment of cyanide, is still needed. Cyanase converts cyanate to CO2 and NH3, but its industrial practicality is limited because the reaction requires HCO3− as a substrate. In this study, we used carbonic anhydrase from Sulfurihydrogenibium azorense (SazCA) to provide HCO3− for cyanase from Thermomyces lanuginosus (TlCyn); both TlCyn and SazCA were purified by one-step heating without prior cell lysis. The heat treatment resulted in higher activities of both enzymes than the conventional two-step process. From a 50 mL-culture, the highest total activity of 147 U and 47,174 WAU was obtained from 5 min of heating at 60 and 80 °C for TlCyn and SazCA, respectively. The coupled enzymatic system was used to degrade cyanate in three different matrices: 50 mM Tris-HCl (pH 8), industrial wastewater, and artificial wastewater. In the industrial wastewater, with the addition of 0.75 WAU (Wilbur-Anderson unit) of SazCA, cyanate degradation using 0.5 mM NaHCO3 was similar to that using 3 mM NaHCO3, indicating an 83% reduction in NaHCO3. We have demonstrated that the dependence on HCO3− of cyanate degradation can be effectively alleviated by using low-cost heat-purified TlCyn and SazCA; the industrial practicality of the coupled enzymatic system is therefore improved. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CARBONIC anhydrase
*INDUSTRIAL wastes
*SEWAGE
*ENZYMES
*POISONS
Subjects
Details
- Language :
- English
- ISSN :
- 20734344
- Volume :
- 13
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Catalysts (2073-4344)
- Publication Type :
- Academic Journal
- Accession number :
- 161437849
- Full Text :
- https://doi.org/10.3390/catal13010076