草果三种真菌性叶部病害病原鉴定及生物学特性测定.

【Objective】 This paper aimed to study the pathogen and biological characteristics of three leaf diseases of Amomum tsaoko Crevost et Lemarie in Yunnan province, and to provide theoretical basis for the control of leaf spot of A.tsaoko. 【Method】Three fungal diseases were discovered from A.tsaoko plantation in Nujiang Prefecture, Yunnan province. Three strains, including CG-N01, CG-N02 and CG-N03 were obtained by single spore culture, their pathogenicity was determined, spore morphology was observed according to morphological characteristics, and pathogen was identified by phylogenetic tree constructed by multi-gene sequence analysis (rDNA-ITS, tub2, LSU and tef-1 α), and biological characteristics were determined. 【Result】 The morphological characteristics of CG-N01, CG-N02 and CG-N03 were in accordance with the typical characters of Pyrus costina, Phoma matteuccola and Colletotrichum mondsii, respectively. The rDNA-ITS, LSU and tef-1 α sequences of CG-N01 were 489, 901 and 475 bp, respectively ( the login numbers were MN596209, MN689811 and MN696687, respectively) ; The rDNA-ITS, LSU and tef-1 α sequences of the strain CG-N02 were 517, 997 and 529 bp respectively (the login numbers were MN596212, MN650644 and MN696686, respectively) ; The rDNA-ITS, tub2 and tef-1 α sequences of strain CG-N03 were 560, 926 and 738 bp, respectively ( the login numbers were MN596210, MN650645 and MN696688, respectively). Purified strains of CG-N01, CG-N02 and CG-N03 had high homology with P. costina, P. matteuccola and C. simmondsii, respectively. Biological determination showed that strain CG-N01 was advantageous to mycelium growth and conidia formation under the conditions of 28 ℃, alternating light and dark, and pH 6.0. Strains CG-N02 and CG-N03 were favorable for sporulation at 25 ℃, alternating light and darkness, and pH 8.0. 【Conclusion】 This study first reported that C. simmondsii can infect and cause Colletotrichum gloeosporioides, the biological characteristics of the pathogens of leaf spot, leaf blast and anthracnose were determined to provide scientific basis and theory support for the diversity study and effective control of the disease. [ABSTRACT FROM AUTHOR]