miR-582-5p 调控 Notch1 减轻心肌缺血再灌注损伤的研究.

Objective　To investigate the role of miR-582-5p in regulating Notch1 to protect myocardium， by detecting the expression changes of miR-582-5p and Notch1 after myocardial ischemia reperfusion and HR model of HL-1 cells in mice. Methods　 The mouse myocardial ischemia-reperfusion model and HL-1 cell OGD reoxygenation model were established. The expression of miR-582-5p and Notch1 in mouse IR and cell HR was detected by qPCR，and then the targeted binding sites of miR-582-5p and Notch1 were predicted by TargetScan. Inhibitor and mimics of miR-582-5p and Notch1 were transfected into cells，and the expression of miR-582-5p and Notch1 was detected again. Finally，CCK-8 assay was used to detect the cell viability of HL-1 cells transfected with inhibitor and mimics. Results　 After HR，the expression of miR-582-5p was significantly increased （P < 0.01） and the expression of Notch1 was significantly decreased in IR mouse and HL-1 cells （P < 0.001） . After transfection of inhibitor，the expression of miR-582-5p was significantly down-regulated （P < 0.001），while Notch1 was significantly up-regulated （P = 0.017），and the viability of cardiomyocytes was significantly increased （P = 0.006）， but the result was opposite after transfection of mimics. Conclusion　 miR-582-5p can protect myocardium against myocardial ischemia and hypoxia by regulating Notch1. [ABSTRACT FROM AUTHOR]