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miR-582-5p 调控 Notch1 减轻心肌缺血再灌注损伤的研究.
- Source :
-
Journal of Kunming Medical University / Kunming Yike Daxue Xuebao . 2022, Vol. 43 Issue 12, p12-17. 6p. - Publication Year :
- 2022
-
Abstract
- Objective To investigate the role of miR-582-5p in regulating Notch1 to protect myocardium, by detecting the expression changes of miR-582-5p and Notch1 after myocardial ischemia reperfusion and HR model of HL-1 cells in mice. Methods The mouse myocardial ischemia-reperfusion model and HL-1 cell OGD reoxygenation model were established. The expression of miR-582-5p and Notch1 in mouse IR and cell HR was detected by qPCR,and then the targeted binding sites of miR-582-5p and Notch1 were predicted by TargetScan. Inhibitor and mimics of miR-582-5p and Notch1 were transfected into cells,and the expression of miR-582-5p and Notch1 was detected again. Finally,CCK-8 assay was used to detect the cell viability of HL-1 cells transfected with inhibitor and mimics. Results After HR,the expression of miR-582-5p was significantly increased (P < 0.01) and the expression of Notch1 was significantly decreased in IR mouse and HL-1 cells (P < 0.001) . After transfection of inhibitor,the expression of miR-582-5p was significantly down-regulated (P < 0.001),while Notch1 was significantly up-regulated (P = 0.017),and the viability of cardiomyocytes was significantly increased (P = 0.006), but the result was opposite after transfection of mimics. Conclusion miR-582-5p can protect myocardium against myocardial ischemia and hypoxia by regulating Notch1. [ABSTRACT FROM AUTHOR]
Details
- Language :
- Chinese
- ISSN :
- 2095610X
- Volume :
- 43
- Issue :
- 12
- Database :
- Academic Search Index
- Journal :
- Journal of Kunming Medical University / Kunming Yike Daxue Xuebao
- Publication Type :
- Academic Journal
- Accession number :
- 161318069
- Full Text :
- https://doi.org/10.12259/j.issn.2095-610X.S20221207