黑果枸杞茎叶响应 NaCl 胁迫合成花色苷的转录组学 分析.

In order to analyze the process of Lycium ruthenicum Murr.（L. ruthenicum）shoots in anthocyanin biosynthesis response to salt stress，L. ruthenicum seedlings were treated with 300 mmol/L NaCl，and the shoots were collected at 3 d after treatment，and the anthocyanin content and transcriptome were analyzed by LC-MS/MS and RNA-seq. Combined with the analysis of species and contents of anthocyanin and differential expressed genes（DEGs）in GO and KEGG pathway，DEGs related to anthocyanin biosynthesis induced by salt stress were mined，and the results of transcriptome data were verified by RT-qPCR. The results showed delphinidin-3-O-rutinoside in L. ruthenicum shoots was the highest upregulated anthocyanin（11.9 times），and the content of petunia-3-O-rutinoside was the highest anthocyanin（5.313±0.286）μg/g. Total 1 416 DEGs（P<0.01）were screened，including 867 up-regulated and 549 down-regulated DEGs， which were classified into GO terms，such as catalytic activity，photosystem and single-organism metabolic process. DEGs were significantly enriched in 14 KEGG pathways. Meanwhile，there were 7 DEGs involved in anthocyanin biosynthesis and all of them were up-regulated，and 27 DEGs were involved in phytohormone signal transduction，in which 15 DEGs were related to ABA signal transduction pathway. The expressions of 11 MYB and 7 bHLH transcription factors were significantly changed. Sum up，the results of this study suggested that DEGs related to ABA pathway，MYB，bHLH transcription factor and anthocyanin biosynthesis play important role in anthocyanin biosynthesis in L.ruthenicum shoot responses to NaCl stress. [ABSTRACT FROM AUTHOR]