原核生物转录调控研究技术及进展.

The regulation of gene expression in prokaryotes mainly occurs at the transcriptional level. Knowledge on the regulation of transcription in prokaryotes is conducive to understanding the mechanism of gene expression regulation. In recent years，with the breakthrough of molecular biology and related technologies，the research technology of transcriptional regulation has also been continuously developed. The technical methods and new developments of prokaryotic transcriptional regulation are reviewed in the present study，including electrophoretic mobility shift assay，DNase I footprinting technology，chromatin immunoprecipitation technology，microthermophoresis technology， isothermal titration calorimetry and bacterial one hybrid system，aiming to systematically understand the advantages/disadvantages and suitability of these methods，help researchers to better use the transcriptional regulation of prokaryotes for bringing great benefits to human life. [ABSTRACT FROM AUTHOR]