Iron nanoparticles induced the growth and physio-chemical changes in Kobresia capillifolia seedlings.

Iron nanoparticles (NPs) priming is known to affect the seed germination and seedling growth in many plants. However, whether it has an important role in stimulating the growth of perennial Qinghai-Tibet Plateau plants remains unclear. In this study, the effects of seed priming with different concentrations of nFe 2 O 3 and FeCl 3 (10, 50, 100, 500, and 1000 mg L−1) on seed germination, plant growth, photosystem, antioxidant enzyme activities, root morphology, and biomass distribution of Kobresia capillifolia were evaluated under laboratory conditions. The results showed that compared with treatment materials, concentration had more significant effects on K. capillifolia development. There was no significant impact on germination rate were discovered under all treatments, but decreased the seed mildew rate at 100 mg L−1 nFe 2 O 3. Compare with control, Fe-based priming significantly decreased root biomass. All Fe-based treatments increased rubisco activity of leaves, and significantly enhanced Pn at ranged from 10 to 100 mg L−1. Meanwhile, chlorophyll contents were decreased, the chloroplasts were swollen, and thylakoids were disorganized under all Fe treatments. Iron-based priming significantly enhanced SOD, POD, and CAT activities in Kobresia roots. In conclusion, the thick cuticle-covered seed coat of K. capillifolia postponed the penetration of FeNPs into seeds, so FeNPs priming had a weak impact on seed germination. The sustainable release of Fe ions from FeNPs and the uptake of Fe ions by roots affected the physiology, biochemistry and morphology of K. capillifolia. The findings of this study provide an in-depth understanding of how FeNPs impact the alpine meadow plant, K. capillifolia. [Display omitted] • Fe 2 O 3 NPs had no significant directly effect on Kobresia capillifolia seed germination. • High concentration Fe 2 O 3 NPs disrupted chloroplast structural and reduced photosynthesis. • Fe 2 O 3 NPs induced oxidative stress response in Kobresia capillifolia. • Fe 2 O 3 NPs had negatively affected roots more than leaves in Kobresia capillifolia. • Fe 2 O 3 NPs reduced biomass via inhibiting roots development and substance translocation. [ABSTRACT FROM AUTHOR]