Back to Search
Start Over
Quebrachitol from Putranjiva roxburghii Wall. (Putranjivaceae) a potent antimalarial: Pre-clinical efficacy and its interaction with PfLDH.
- Source :
-
Parasitology International . Feb2023, Vol. 92, pN.PAG-N.PAG. 1p. - Publication Year :
- 2023
-
Abstract
- Researchers are exploring natural resources in search of a new and effective anti-malarial compound to address the challenges in malarial treatment due to emerging incidences of drug-resistant strains. Following background knowledge of traditional medicine, we evaluated the in-vitro and in-vivo anti-malarial efficacy of Putranjiva P. roxburghii (Putranjivaceae) twigs ethanol extracts and fraction (PRT). In-vitro parasite-specific lactate dehydrogenase (pLDH) assay was performed using a chloroquine-sensitive Plasmodium falciparum strain. The results of the in-vitro study were further validated by in-vivo anti-malarial studies on P. berghei Keyberg 173 (K173) infected mice. The crude ethanol extract of the PRT showed the most moderate antiparasitic activity (IC 50 = 15.51 μg/mL). In contrast, its butanol fraction extract showed potent activity (IC 50 = 5.14 μg/mL) with a selectivity index (SI) of 28.87. Two phytochemicals, viz. 2, 4 dihydroxy-5-(hydroxymethyl) benzoic acid (DHMBA), and quebrachitol (QBC), were identified with anti-parasitic activity (IC 50 = 5.01 μg/mL and 0.87 μg/mL) and selectivity index (SI) of 45 and 158. The in-vivo studies confirmed the significant anti-malarial activity of QBC at the dose of 30 and 60 mg/kg body weight with chemo-suppression values of 73.26% and 61.88%, respectively. The present study demonstrates the bioactive marker-based standardization of P. roxburghii twig, the antiplasmodial potential of PRT , and the role of QBC in suppressing parasitemia. The findings of the study support QBC as a prospective lead for a natural product-based adjunct remedy to conventional antiparasitic agents for malarial infectious. [Display omitted] • First report which demonstrates anti-plasmodial potential new compounds from P. roxburghii twig • In-vito and in-vivo assays showed antimalarial of potential of 2, 4 dihydroxy-5-(hydroxymethyl) benzoic acid (DHMBA) and quebrachitol (QBC) • QBC significantly inhibited the in-vitro growth of P. falciparum (IC 50 = 0.87 ± 0.12 μg/mL) • QBC (30 mg/kg) resulted in chemosupression (73.26%) in P. berghei infected mice and improved survival time. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13835769
- Volume :
- 92
- Database :
- Academic Search Index
- Journal :
- Parasitology International
- Publication Type :
- Academic Journal
- Accession number :
- 161016248
- Full Text :
- https://doi.org/10.1016/j.parint.2022.102675