五味子甲素通过 MAPK/ ERK 途径拮抗黑素细胞中 黑素生成的机制研究.

OBJECTIVE: To probe into the mechanism of schisandrin A antagonizes melanogenesis in melanocytes through mitogen-activated protein kinase (MAPK) / extracellular signal-regulated kinase (ERK) pathway. METHODS: The human epidermal melanocyte group, schizandrin A low, medium and high dose group (125, 250, 500 μmol / mL) were set up. After incubation, cell viability was measured by MTT method, apoptosis indicators were evaluated by flow cytometry, and melanin preparation standard curve was used to measure melanin synthesis ability, enzyme linked immunosorbent assay was used to measure the level of tyrosinase, the mRNA and protein expression levels of MAPK and ERK were determined by reverse transcription-polymerase chain reaction and Western blotting. RESULTS: Compared with the human epidermal melanocyte group, the OD value, survival rate, melanin synthesis level and tyrosinase level of schizandrin A low, medium and high dose group decreased, with statistically significant differences (P< 0. 05). With the increasing dose of schizandrin A, the OD value, survival rate, melanin synthesis level and tyrosinase level of human epidermal melanocytes decreased, and the effect of each dose group of schisandrin A showed the dose-effect trend (P < 0. 05). Compared with the human epidermal melanocyte group, the apoptotic rate, the mRNA and protein expression levels of MAPK, ERK in the schisandrin A low, medium and high dose group increased, with statistically significant differences (P < 0. 05). With the increasing dose of schizandrin A, the apoptosis rate, the mRNA and protein expression levels of MAPK, ERK of human epidermal melanocytes increased, and the effects of each dose group of schisandrin A showed the dose-effect trend (P < 0. 05). CONCLUSIONS: Schizandrin A can inhibit the proliferation of melanocytes, promote the apoptosis of melanocytes, and then inhibit the melanogenesis. Its mechanism may be related to the activation of MAPK/ ERK pathway by promoting the mRNA and protein expression levels of MAPK and ERK in human epidermal melanocytes by schizandrin A. [ABSTRACT FROM AUTHOR]