卷丹新种质JD-h-15 的形态特征与遗传变异分析.

In order to cultivate a high-quality new germplasm of Lilium lancifolium, JD-h-15 induced by colchicine was used to analyze the chromosome ploidy after 3 years’ planting in the field. The plant morphology, stigma and pollen fertility, leaf epidermis and starch grain characteristics, and active components of bulb were compared in JD-h-15 and L. lancifolium control. Further their difference on molecular levels was analyzed by ISSR marker technology. The results showed that JD-h-15 has restored to be a triploid plant from an aneuploid chimera before. The plant height, leaf interval and leaf area of JD-h-15 significantly increased compared with that in the control, as well as the stem diameter and leaf number per plant decreased, in addition the flowering period per plant was advanced in JD-h-15 by 14 d. The single head rate of bulb in JD-h-15 was 93.75%, significantly higher than 70.00% of that in the control. Analysis of stigma acceptability in different flower periods revealed the stigma in 50-60 mm flower bud of JD-h-15 had a higher acceptability than that in the control. 13.71% of the pollen for JDh-15 germinated normally, while the control pollen did not germinate, which was consistent with the expansion of ovary in JD-h-15 after self-pollination. Micromorphological analysis showed that the pollen grains of JD-h-15 were larger than those of the control, while the starch grains in bulbils and bulbs were much smaller than those of the control. In JD-h-15 bulbs, the contents of soluble sugar, total flavonoids and total saponins increased by 42.37%, 67.54% and 45.65% respectively, while ascorbic acid, total phenolic acids and total alkaloids decreased by 22.86%, 33.02% and 50.41% compared with that in the control. The 3 years’ old JD-h-15 and the control plants were genetically stable after ISSR marker analysis. Compared with the control, JD-h-15 plants experienced the genetic variation and the variation rate of JD-h-15 was 21.30%. These data provided a reference for the improvement of L. lancifolium genetic characters and the cultivation of new germplasm used by colchicine in lilies. [ABSTRACT FROM AUTHOR]