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Amperometric biosensors based on alcohol oxidase and peroxidase–like nanozymes for ethanol determination.

Amperometric biosensors based on alcohol oxidase and peroxidase–like nanozymes for ethanol determination.

Authors :
Stasyuk, Nataliya
Demkiv, Olha
Gayda, Galina
Zakalska, Oksana
Nogala, Wojciech
Gonchar, Mykhailo
Source :
Microchimica Acta. Dec2022, Vol. 189 Issue 12, p1-10. 10p.
Publication Year :
2022

Abstract

The aim of the current research is to design alcohol oxidase-based amperometric biosensors (ABSs) using hybrid metallic nanoparticles as artificial peroxidases (PO) or PO-like nanozymes (NZs). A lot of metallic PO-like NZs were synthesized and tested with respect to their ability to substitute natural PO in solution and on amperometric electrode. The most effective PO mimetics were coupled with alcohol oxidase (AOX) on graphite electrodes (GEs) and characterized. Two types of modified GEs, namely, the AOX/nAuCePt/GE and the AOX/nFePtAu/GE show the highest sensitivities to ethanol (2600 A⋅M−1⋅m−2 and 1250 A⋅M−1⋅m−2, respectively), low limits of detection (1.5 µM and 2.2 µM), broad linear ranges (5 – 100 µM and 12 – 120 µM), as well as satisfactory storage stabilities. The most sensitive bioelectrode AOX/nAuCePt/GE was used as ABS for ethanol determination in real samples. The practical feasibility of the constructed ABS was demonstrated by determination of ethanol in beverages, human blood and saliva. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00263672
Volume :
189
Issue :
12
Database :
Academic Search Index
Journal :
Microchimica Acta
Publication Type :
Academic Journal
Accession number :
160907963
Full Text :
https://doi.org/10.1007/s00604-022-05568-z