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Quantitative proteomic analysis to understand the role of Arabidopsis thaliana LEAFY COTYLEDON 2 in promoting lipid accumulation in Chlorella sorokiniana by upregulating photosynthetic proteins and G3PDH.
- Source :
-
Journal of Applied Phycology . Dec2022, Vol. 34 Issue 6, p3035-3046. 12p. - Publication Year :
- 2022
-
Abstract
- Microalgae have attracted significant attention for biofuel production and transcription factors that can effectively regulate lipid accumulation in higher plants. In this study we expressed the transcription factor LEAFY COTYLEDON 2 from Arabidopsis thaliana in Chlorella sorokiniana. The relative electron transport rate (rel.ETR) of C. sorokiniana-AtLEC2 improved significantly under high-intensity light. As a result, the total lipid content and main fatty acid composition (C16, C18) increased by more than onefold. Differentially expressed proteins were analyzed using a label-free strategy to elucidate the regulation mechanism of AtLEC2. The results showed upregulation of TAG biosynthesis (G3PDH) and photosynthesis-related proteins (e.g., ATP synthase F0, Photosystem II, Photosystem I P700, cytochrome b6, cytochrome b6f, chlorophyll a-b binding protein, Photosystem I assembly protein). Furthermore, AtLEC2 expression in C. sorokiniana led to fixed carbon flow in photosynthesis to TAG through G3PDH upregulation. The current study will provide a new direction for the cultivation of high-yield oil algae and will reveal the mechanism of regulation of AtLEC2 on TAG accumulation in C. sorokiniana. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09218971
- Volume :
- 34
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Journal of Applied Phycology
- Publication Type :
- Academic Journal
- Accession number :
- 160778521
- Full Text :
- https://doi.org/10.1007/s10811-022-02833-9