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Myomaker and Myomixer Characterization in Gilthead Sea Bream under Different Myogenesis Conditions.

Authors :
Perelló-Amorós, Miquel
Otero-Tarrazón, Aitor
Jorge-Pedraza, Violeta
García-Pérez, Isabel
Sánchez-Moya, Albert
Gabillard, Jean-Charles
Moshayedi, Fatemeh
Navarro, Isabel
Capilla, Encarnación
Fernández-Borràs, Jaume
Blasco, Josefina
Chillarón, Josep
García de la serrana, Daniel
Gutiérrez, Joaquim
Source :
International Journal of Molecular Sciences. Dec2022, Vol. 23 Issue 23, p14639. 18p.
Publication Year :
2022

Abstract

Skeletal muscle is formed by multinucleated myofibers originated by waves of hyperplasia and hypertrophy during myogenesis. Tissue damage triggers a regeneration process including new myogenesis and muscular remodeling. During myogenesis, the fusion of myoblasts is a key step that requires different genes' expression, including the fusogens myomaker and myomixer. The present work aimed to characterize these proteins in gilthead sea bream and their possible role in in vitro myogenesis, at different fish ages and during muscle regeneration after induced tissue injury. Myomaker is a transmembrane protein highly conserved among vertebrates, whereas Myomixer is a micropeptide that is moderately conserved. myomaker expression is restricted to skeletal muscle, while the expression of myomixer is more ubiquitous. In primary myocytes culture, myomaker and myomixer expression peaked at day 6 and day 8, respectively. During regeneration, the expression of both fusogens and all the myogenic regulatory factors showed a peak after 16 days post-injury. Moreover, myomaker and myomixer were present at different ages, but in fingerlings there were significantly higher transcript levels than in juveniles or adult fish. Overall, Myomaker and Myomixer are valuable markers of muscle growth that together with other regulatory molecules can provide a deeper understanding of myogenesis regulation in fish. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
16616596
Volume :
23
Issue :
23
Database :
Academic Search Index
Journal :
International Journal of Molecular Sciences
Publication Type :
Academic Journal
Accession number :
160740331
Full Text :
https://doi.org/10.3390/ijms232314639