Antibacterial peptide encapsulation and sustained release from chitosan-based delivery system.

[Display omitted] • Chitosan-based nanoparticles were prepared for peptide encapsulation. • The loading rate of nanoparticles to antimicrobial peptides was over 80% • Nanoparticles loaded with antimicrobial peptides can prolong the antibacterial time. • Simple operation, non-toxic and harmless, realize the principle of green chemistry. Antimicrobial peptides (AMPs) are a class of short polypeptides that are considered to be attractive future alternatives to traditional antibiotics for their wide sources, excellent antimicrobial properties and low drug resistance. However, AMPs are easily degraded, causing unstable antibacterial properties and a short effective time. Nanocarriers with good biocompatibility can improve the stability of AMPs, achieve sustained and stable release, and improve the bioavailability of AMPs. Herein, composite nanoparticles (NPs) prepared by ionic interaction between chitosan quaternary ammonium salt (HACC) and sodium dodecyl sulfate (SDS) were used as carriers for AMPs. The loading rate of NPs to AMP was over 80%. The AMP loaded in the particles had a longer antibacterial activity than the free AMP, and the NPs had good cell compatibility. The preparation method of particles is simple, pollution-free, and compound green chemistry concept. Moreover, it is convenient to load into various forms of biomedical materials, which is a promising carrier. [ABSTRACT FROM AUTHOR]