Electric yo-yo centrifugation combining with paper-based microfluidic immunoassay chip for inflammatory biomarkers detection in whole blood.

At present, most countries or regions use commercial centrifuges for centrifugation, but this is out of reaching for limited-resource areas. To overcome this problem, a portable electric yo-yo as centrifuge was firstly proposed to obtain serum, and this device can be combined with paper-based analytical devices for enzyme-linked immunosorbent assay (ELISA) analysis from human whole blood. In this study, inflammatory biomarkers C-reactive protein (CRP) and serum amyloid A (SAA) were used as target biomarker to verify the performance of the proposed method. The results shows good performance and their detection limits were determined to be 580 pg/mL for CRP and 800 pg/mL for SAA, respectively. We believe this method provides a new platform of low cost and fast detection for inflammatory biomarkers in the limited-resource settings. Using electric yo-yo device to implement inflammatory biomarkers detection in whole blood paper-based microfluidic immunoassay chip. [Display omitted] • A low-cost, portable and effective electric yo-yo centrifugal device is firstly proposed for blood centrifugation. • Paper-based microfluidic immunoassay chip used for inflammatory biomarkers analysis in whole blood. • The method shows good potential to on-site/limited-resource testing. [ABSTRACT FROM AUTHOR]