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Research Note: Creatine monohydrate alleviates protein breakdown induced by corticosterone via inhibiting ubiquitin proteasome pathway in chicken myotubes.

Authors :
Sun, Mingfa
Jiao, Hongchao
Zhao, Jingpeng
Wang, Xiaojuan
Li, Haifang
Zhou, Yunlei
Lin, Hai
Source :
Poultry Science. Dec2022, Vol. 101 Issue 12, pN.PAG-N.PAG. 1p.
Publication Year :
2022

Abstract

Stress is a common problem diminishing the muscle development of broilers. Creatine (Cr), an energy buffer in skeletal muscle, plays a fundamental role in muscle physiology. This study aimed to evaluate the effect of Cr monohydrate (CMH) on protein breakdown in chicken myotubes challenged by corticosterone (CORT) in vitro. The morphology of myotube was measured and the activation of ubiquitin proteasome (UP) pathway was determined. The result showed that CORT treatment decreased myotube diameter (P < 0.05), increased 3-methyl-histidine (3M-His) content in medium, enhanced the mRNA expression levels of muscle ring finger1 (MuRF1) and Atrogin1 (P < 0.001), and Atrogin1 protein level (P < 0.05) compared with control. By contrast, CMH increased myotube diameter (P < 0.05) and myosin heavy chain (MHC) expression (P < 0.001), whereas decreased 3M-His and the mRNA and protein levels of Atrogin1 (P < 0.05), compared to control. In the present of CMH, the decreased myotube diameter and increased 3M-His, mRNA levels of MuRF1 and Atrogin1 , and Atrogin1 protein level by CORT were partially relieved (P < 0.05). Hence, the result suggests that CMH alleviates CORT-induced protein breakdown by suppressing Atrogin1 expression in chicken myotubes. The result highlights the potential application of CMH in regulating muscle protein catabolism in chickens under stress. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00325791
Volume :
101
Issue :
12
Database :
Academic Search Index
Journal :
Poultry Science
Publication Type :
Academic Journal
Accession number :
160399499
Full Text :
https://doi.org/10.1016/j.psj.2022.102177