In-Vitro Antimicrobial, Antioxidant and Enzyme Inhibitory Activities of Fixed Oil Extracted from Stem Bark of Tamarix aphylla.

This research work was aimed to assess the antioxidant, antimicrobial and enzyme inhibitory properties of fixed oil extracted from Tamarix aphylla stem bark. Fixed oil composition was assessed using gas chromatography–mass spectrometry (GC-MS) technique. The plant extract activity was assessed against various bacterial and fungal strains using hole diffusion and macro dilution techniques. Spectrophotometric method was used to measure the lipoxygenase and acetylcholinesterase inhibitory activities while antioxidant activity was assessed using ABTS radical scavenging and FRAP assay. Fixed oils were analyzed with the help of GC-MS, resulting in the identification of 03 compounds. Antibacterial assay showed that Staphylococcus aureus was the most affected bacterial strain with 7 mm zone of inhibition while among fungal strains, Candida glabrata was most significantly inhibited with 11 mm zone of inhibition. The tested samples were very efficient in its inhibitory activities against bacteria when 0.125, 0.5 and 1.0 mg/ml concentration was used. Similarly in case of fungi, 0.5, 1.0, 2.0 and 4.0 mg/ml of tested samples were showing significant results. Lipoxygenase and acetylcholinesterase inhibitory activities showed IC50 values of 90.1 μg/mL and 128.4 μg/mL, respectively. The antioxidant activity was assessed using ABTS radical scavenging and FRAP assays, and maximum inhibition of 87.01% was achieved at 800 μg/mL concentration. These results validate and endorse the centuries old folklore usage of T. aphylla as medicinal plant against various diseases. [ABSTRACT FROM AUTHOR]