Photochemical study of flavonoid "isovitexin" present in the whole plant Anastatica hieroch untica L. cultivated in Iraq.

This study detects the presence of an important flavonoids "isovitexin" present in the whole plant Anastitica hierochuntica L. cultivated in Iraq. The absence of any study concerning the isovitexin content of this medicinal plant in Iraq and the medicinal importance isovitexin depending on its uses for treatment some disease, acquired this study its value. This study concerned with extraction, identification, isolation, and purification of isovitexin from the whole plant Anastatica hierochuntica L. Extraction of this compound was carried out using two methods. Identification of this compound was done using thin layer chromatography (TLC) where different solvent systems had been tried. This identification was further augmented by using high performance liquid chromatography (HPLC) and then this flavonoid was isolated and purified. The identification of isolated isovitexin was carried out using Melting point (M.P), Thin layer chromatography (TLC), Infrared spectroscopy (IR) and High performance liqude chromatography (HPLC). This study confirms the presence of isovitexin in the whole plant Anastatica hierochuntica L. cultivated in Iraq. Also the result of this study showed that the extraction method NO.2 was the best, because the amount of both extract and isovitexin were higher than from extraction method NO.1. [ABSTRACT FROM AUTHOR]