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DMP8 and 9 regulate HAP2/GCS1 trafficking for the timely acquisition of sperm fusion competence.

Authors :
Wei Wang
Hanxian Xiong
Peng Zhao
Xiongbo Peng
Meng-Xiang Sun
Source :
Proceedings of the National Academy of Sciences of the United States of America. 11/8/2022, Vol. 119 Issue 45, p1-11. 33p.
Publication Year :
2022

Abstract

Sexual reproduction involves the fusion of two gametes of opposite sex. Although the sperm-expressed fusogen HAPLESS 2 (HAP2) or GENERATIVE CELL SPECIFIC 1 (GCS1) plays a vital role in this process in many eukaryotic organisms and an understanding of its regulation is emerging in unicellular systems [J. Zhang et al., Nat. Commun. 12, 4380 (2021); J. F. Pinello et al. Dev. Cell 56, 3380–3392.e9 (2021)], neither HAP2/GCS1 interactors nor mechanisms for delivery and activation at the fusion site are known in multicellular plants. Here, we show that Arabidopsis thaliana HAP2/ GCS1 interacts with two sperm DUF679 membrane proteins (DMP8 and DMP9), which are required for the EGG CELL 1 (EC1)-induced translocation of HAP2/GCS1 from internal storage vesicle to the sperm plasma membrane to ensure successful fertilization. Our studies in Arabidopsis and tobacco provide evidence for a conserved function of DMP8/9-like proteins as HAP2/GCS1 partner in seed plants. Our data suggest that seed plants evolved a DMP8/9-dependent fusogen translocation process to achieve timely acquisition of sperm fusion competence in response to egg cell–derived signals, revealing a previously unknown critical step for successful fertilization. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00278424
Volume :
119
Issue :
45
Database :
Academic Search Index
Journal :
Proceedings of the National Academy of Sciences of the United States of America
Publication Type :
Academic Journal
Accession number :
160244200
Full Text :
https://doi.org/10.1073/pnas.2207608119