文山红河地区苗族人群 20 个常染色体 STR 基因座遗传多态性研究.

Objective To investigate the genetic polymorphisms of 20 autosomal STR loci in Miao population from Wenshan and Honghe regions, and to explore their forensic values. Methods DNA was extracted with the Chelex-100 method. A total of 20 STR loci were amplified from 2 209 healthy unrelated individuals of the Miao population from Wenshan and Honghe regions, Yunnan Province using the PowerPlex®21 System kit. The PCR products were separated by capillary electrophoresis on an ABI 3130Genetic Analyzer, and alleles were genotyped by GeneMapper ID (Version 3.2) software. Forensic parameters were calculated by Modified-Powerstats software, and the Hardy-Weinberg equilibrium (HWE) test was performed. The Fst values and Nei's genetic distance were calculated by Arlequin and Phylip software, respectively. Multidimensional scaling (MDS) analysis was performed with SPSS v24.0 statistical software and the neighbor-joining (N-J) phylogenetic tree was constructed using the MEGA v6.0 software. Results A total of 265 alleles and 1 080 genotypes were observed. The allele frequency of the 20 STRs ranged from 0.000 2 to 0.5718. No deviation from Hardy-Weinberg equilibrium (P > 0.05/20 = 0.002 5) was observed. The cumulative discrimination power (CDP) and cumulative probability of exclusion (CPE) with 20 STRs were 0.999 999 999 999 999 999 999 932 674 151 and 0.999 999 935 545 335, respectively. Population comparison showed that Wenshan and Honghe Miao nationality was genetically closer to Yunnan Vietnamese. Conclusion These 23 STR loci are highly genetic polymorphic and informative in the Miao population from Wenshan and Honghe regions, Yunnan Province, which can be used for forensic identification and population genetic research. [ABSTRACT FROM AUTHOR]