mTOR 在 SD 大鼠 TMJOA 髁突软骨中表达变化的实验研究.

Objective To investigate the expression of mTOR in TMJOA condylar cartilage of SD rats. Methods The model of TMJOA in SD rats was established by unilateral mastication，and the corresponding shamoperation group was established. TMJOA model was established by unilateral mastication.The right condyle was taken 2, 4 and 8 weeks after modeling. HE staining and safranine o-solid green staining were used for pathological examination； The expressions of Sox9 and mTOR were detected by immunohistochemical staining. Results　Hematoxylin-eosin (HE) staining and saffron o-green staining showed that the condylar cartilage of the control group had clear structure，smooth and intact surface，and the proteoglycans were evenly distributed. In the experimental group (TMD group)，obvious degenerative changes of condylar cartilage were observed over time (P < 0.05)，and the lesions were the highest in 4 W. Immunohistochemical staining showed that the expression level of Sox9 in the condylar cartilage of the experimental group decreased successively at 2 W (P < 0.01)，4 W (P < 0.05) and 8 W (P < 0.05)，and were lower than that of the control group. The expression level of mTOR decreased at 2 W (P < 0.05) and 4 W (P < 0.001)，but increased relatively at 8 W (P < 0.05) . Conclusion　With the progress of inflammation，mTOR in TMJOA condylar cartilage of SD rats shows a trend of early down-regulation and late upregulation, which plays a key role in transforming protective autophagy into destructive apoptosis. [ABSTRACT FROM AUTHOR]